Single-chain variable fragments (scFvs) are essential building blocks for breakthrough therapeutics including bispecific antibodies and CAR-T cell therapies. They are composed of the variable heavy (VH) and variable light (VL) domains joined by a flexible peptide linker.
scFvs retain antigen-binding activity, are straightforward to engineer, and can be readily fused to other proteins. Owing to their small size, they often show improved tissue penetration when used alone, although they also have a shorter half-life.
However, the absence of constant domains increases their tendency to aggregate and misfold, which can make bispecific formats containing scFvs less stable – leading to:
High development costs
Poor therapeutic performance
Manufacturing challenges and reduced yields
Project cancellations
For three decades, the field has lacked a broadly applicable solution to the scFv stability problem. Until now.
Optimized CHO Expression for Challenging Antibody Formats
evitria now offers Opti-mAb® technology from AAX Biotech – a proprietary molecular stabilization strategy for scFvs.
This significantly improves stability of scFvs and scFv-containing bispecific antibodies. The result: higher titers, improved quality, and consistent manufacturability for even the most complex antibody constructs.
“Opti-mAb® represents a significant advancement in scFv development. By combining AAX Biotech’s proprietary optimization with our 15 years of exclusive CHO expertise, we can now deliver exceptional results for constructs that previously challenged conventional platforms.”
Download our comprehensive technology spotlight about Opti-mAb® to see how our strategic partnership with AAX Biotech AB provides early access to the Opti-mAb® technology, allowing you to address key developability risks, eliminate aggregation, and significantly increase High-Throughput expression yields for challenging scFv constructs.
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“We have been working with evitria for several years and highly appreciate the high consistency of the delivered material.”
Sr. Scientist, Biotherapeutics, Switzerland
“We have been working with evitria since 2022. We appreciate the openness and flexibility of the team, the high speed and quality of the proteins they deliver.”
Radiopharm Theranostics Ltd.
“We are happy paying for the assured quality that evitria brings.”
Sr Scientist, Biotherapeutics, Series B Startup, Boston
The evitria + AAX Biotech Partnership
evitria combines 15+ years of exclusive CHO transient expertise with AAX Biotech’s cutting-edge Opti-mAb® technology for scFv stabilization.
This partnership enables you to access proprietary optimization while maintaining the absolute scientific flexibility, Swiss precision, and comprehensive quality standards that define evitria’s service model.
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FAQs about Opti-mAb® Technology
Opti-mAb® typically delivers 2-40x higher expression titers compared to non-optimized scFvs. This makes it particularly valuable for challenging constructs that yield poorly in standard platforms or require large quantities for in vivo studies.
Yes. Opti-mAb® acts at the format design stage and creates optimized scFv sequences that are fully compatible with industry-standard expression systems, such as CHO cells. This ensures that the candidates identified during discovery can be seamlessly transitioned to clinical-scale manufacturing without requiring further re-engineering or facing comparability issues.
Opti-mAb® can be used for any scFvs- based formats, including complex bispecifics in any orientation, fusion proteins and stand-alone scFvs.
Yes. Expedited timelines are available for urgent projects. Contact our team to discuss your specific timeline requirements and we’ll design a production strategy to meet your milestones.
Absolutely. Unlike providers with rigid packages, we individually discuss and design analytical strategies to match your specific needs – from basic QC to comprehensive characterization including mass spectrometry, glycan profiling, and functional assays.
Let’s get in touch with our experts now!
Our team of experts looks forward to answering all your concerns regarding our services and solutions.
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